Ph.D. Pavle Pavlovic (director)

» PRECLINICAL STUDIES: Anticancer substancesExperimental models: Human cancer cell lines: glioma
» neuroblastoma
» melanoma
» smalland non small lung cells
» colon
» ovarian etc. Tumor biopsies Animal models of - brain tumor (rat 9L glioma model)Melanom (mouse B16 melanom)Methods and assays: Antiproliferative assays (BrdU)Citotoxicity assays (MTT
» NRU
» SRB)Cell cycle analysis (FACS)Assays of apoptosis induction-morphological characterization (TUNEL
» PI staining)Nucleosome ELISA assayDNA ladder electrophoresisRT-PCR analysis of apoptosis genesRT-PCR analysis of oncogene expression Fluorocytometric analysis of cell cycle arrest and apoptosisImmuno assays (ELISA) of oncogene products Western blot analysis of cancer cell proteinsOther substances (neurobiology: anticonvulsive
» antipsychotic drugs
» etc.) Experimental models: Primary cultures of neurones Primary cultures of astrocytes Animal models of: Parkinsons disease (6-OH lesion of rat striatum) Alzheimer disease Brain injury (rat cortical lesion) Experimental pschyosis Methods and assays Real-Time PCR analysis of gene expressionIn situ hybridizationImmunohistochemistry Electrophysiological monitoring Behavioral testsII HUMAN MEDICINEMOLECULAR DIAGNOSTICS Use of Real-Time PCR technology for - cancer research for monitoring of various tumor markers- viral and bacterial diagnostics- genetic disease testing Detection of various gene polymorphisms- COLI a1 gene polymorphism in osteoporosis - Y chromosome microdeletions in man with azoospermia MOLECULAR PATHOLOGY TUNEL apoptosis Immunohystology EDUCATION Theoretical and practical courses at the undergraduate and postgraduate level Full mentorship (experimental and theoretical work) for B.Sc
» M.Sc and Ph.D degrees Training in basic experimental techniques of molecular genetics and recombinant DNA technologyTraining in specific techniques for monitoring gene expression Preclinical investigations of immunomodulatory and antineoplastic agents by testing potential immunomodulatory properties of biologically active substances that may have applicability in development of new therapeutic methods in the disorders with immune pathogenesis (autoimmune disorders
» infection and malignancy). Testing of new synthetic pharmacological agents
» or purified biologically active natural products as a potential drugs
» which may be used for therapy of immune-mediated disorders Testing of existing therapeutics
» which may expand their indication to immune-mediated disorders Preclinical investigations of immunomodulatory and antineoplastic agents include examination by adequate experimental models in vivo and in vitro. In vivo investigations of immunomodulatory effects of selected biologically active substances include testing of their immunomodulatory potential in animal models of organ-specific autoimmune diseases as an analogs for human disorders (type 1 diabetes
» multiple sclerosis)
» as well as in models of experimental infections induced by bacteria and their products (endotoxemia). Parameters for evaluation of drug efficacy include: clinical signs of the disease
» histopathological changes
» phenotype characteristics of immune cells
» their proliferative capacity
» and the production of relevant cell-derived mediators (cytokines
» reactive oxygen and nitrogen species). In vitro investigations of immunomodulatory effects of selected biologically active substances include testing at a cellular and molecular level by using normal cells of various origin
» as well as malignant cell lines. Immunological and biological parameters for evaluation of drug efficacy include: cell proliferation
» differentiation
» production and activity of cytokines and their specific antagonists
» apoptosis
» production of reactive oxygen and nitrogen species
» gene expression
» intracellular signaling. Area of expertise and services which can be proposed for foreign market Science field: Plant physiology
» in vitro plant tissue culture of woody (fruit trees
» forest and horticultural species) and herbaceous plants (endemic
» endangered and horticultural species). In vitro multiplication is investigated by androgenesis
» somatic embryogenesis and micropropagation.

biological science

PRECLINICAL STUDIES: Anticancer substances, DNA ladder electrophoresis, neurobiology: anticonvulsive, antipsychotic drugs, HUMAN MEDICINE: molecular diagnostics, molecular pathology, molecular physiology, EDUCATION
ENVIRONMENTAL STUDIES

Prof. Dr Ljiljana Radojeviæ1973-1974. Predoctoral specialization at the University VII, Paris, France (13 months sponsored by University and fellowship of Research Science Funds of Serbia) in the team of Prof. Dr Aries Kovoor. (in vitro culture of woody and herbaceous plants)1977-1978. Postdoctoral training at the University VII, Paris, France (fellowship from Jolliot-Curie Funds, France) in the team of Prof. Dr. A. Kovoor (in vitro culture of woody plants)1980-1985. Four one-month study visits to Gembloux, Belgium, Centre de Recherché Agronomique. Prof. Dr Phillipe Boxus, Head of Department (in vitro culture obtaintion of virus-free plants).1983 and 2000. One month study visit to Gent, Belgium. Faculty of Biology, Prof. Dr. Pierre Debergh (micropropagation of horticultural plants).1977, 1980,1986,1990. Four month of scientific collaboration with Prof. Dr. P. Landre and Prof. A. Benbadis of the Faculty Pierre et Marie Curie, Paris, France. One month study visit to the Faculty Paris-Sud in the La. of Prof. Dr H. Bysseeur.1996-1998. Scientific collaboration (Sabbatical of 18 months) with Prof. Dr Roberto Rodriguez, Faculty of Biology, University of Oviedo, Spain (in vitro culture of Gymnospermeae).2002. Scientific collaboration (sabbatical 3 months) with Prof. Dr Darcy Leal and Prof. Dr Manuel Sanchez, Faculty of Forestry, University of Conception, Chile, (in vitro culture of horticultural woody species).2003. Scientific collaboration (1 month of the cooperation bilaterally: Belgium -Serbia and Montenegro) with Prof. Dr Philipe Druart, Department of Biotechnology, CRA, Gembloux, Belgium.Dr Snezana Zdravkovic-Korac-2001 Scientific collaboration with Prof. Dr Philipe Druart, Department of Biotechnology, CRA, Gembloux, Belgium (genetic transformation of horse chestnut).Mr Sladjana Jevremovic2002,2003, two months cooperation with ) with Prof. Dr Philipe Druart, Department of Biotechnology, CRA, Gembloux, BelgiumIn cooperation with Prof. Dr Philipe Druart, Department of Biotechnology, CRA, Gembloux, Belgium we started preliminary investigation with Pinus nigra and Prunus spp. in project 1573, which enable new proposal for further cooperation. 2. Previous international cooperation Laboratory for ImmunologyDepartment of Medical Biology, Immunology Unit, Faculty of Medicine and Health Sciences, Al Ain, UAEDivision of Infectious Diseases, University of Colorado, Health Science Center, Denver, Colorado, USAHoechst AG, Werk Kalle-ASlbert, Wiesbaden, GermanyDepartment of Biomedical Sciences, University of Catania, ItalyLaboratory of Medicinal Chemistry, North Shore Long Island Jewish Research Institute, New York, USA

2005-11-01 / 2014-05-25